GARCIA CASTILLO VERONICA

Information:

I hold a Ph.D. in Experimental Biology from the Universidad Autónoma Metropolitana-Iztapalapa. I am a member of the National System of Researchers (CONAHCYT) at the Candidate level. My expertise is focused on understanding the molecular processes affected in cancer development and progression, with a particular interest in studying the aberrant metabolism of tumor cells. I analyze the in vitro efficacy of a triple pharmacological therapy that targets the major energy generation pathways in tumor cells, specifically in breast and colorectal cancer phenotypes.

To comprehensively understand the molecular processes impacted in cancer progression, we employ molecular and cellular biology tools. We focus on identifying prognostic markers associated with selective autophagy through proteins such as Atg’s, ULK1, LC3-I and LC3-II, p52, among others. We also study apoptosis through intrinsic caspases (3, 6, and 7), aerobic glycolysis or the Warburg effect via GLUTs, glucose 6-phosphate, phosphoenolpyruvate, pyruvate, LDH-A, among others. Additionally, we investigate DNA damage and repair through PARP-1 and some prognostic markers in the mTOR pathway, a master regulator of cellular energy metabolism.

My methodologies involve in vivo mouse models using BALB/c and athymic FOXN1 strains, cell culture, nucleic acid and protein extraction, qPCR and endpoint PCR, immunohistochemistry, flow cytometry, immunofluorescence, Western Blot, real-time migration and proliferation analysis, and fluorescence microscopy.

Links of Interest: